Corynespora cassiicola is a common pathogen of grasses and other herbaceous plants in the tropics 3,4. Genetic determinism of sensitivity to corynespora cassiicola. Corynespora cassiicola attackes diverse agriculturally important plants, including soybean and cotton, in the us. The present study combines the examination of toxins produced by c. The organism was isolated and identified as corynespora cassiicola according to its morphological characteristics and gene. Corynespora cassiicola from soybean to carbendazim and prothioconazole. A necrotic subcutaneous lesions over the dorsal aspects of both hands with purulent discharge in a patient with chronic subcutaneous infection caused by corynespora cassiicola. Saprophytism and parasitism habits of plants are common survival strategies for this pathogen. Corynespora cassiicola is an important pathogen because its responsible for a considerable loss of crop production and yield. Growth and sporulation of the fungus on several media. Chromone derivatives from a spongederived strain of the. An updated phylogenetic classification of corynespora. Diversity of the cassiicolin gene in corynespora cassiicola. Occurrence of corynespora cassiicola isolates resistant to.
Frequency distribution of ec 50 values for tetramycin against 91 corynespora cassiicola isolates obtained from different cities in shandong, as based on a mycelial growth, b spore germination, and c germ tube elongation at several concentrations. A new foliar disease, corynespora leaf spot, has been. The isolation of um 591 from a patients contact lens indicates the pathogenic potential of this dematiaceous fungus in human. Detection of corynespora cassiicola in soil with realtime. We are setup to allow easy downloads of individual projects and dont provide means to download whole collections of data. These climatic conditions would limit the ability of the pathogen to. Several lineages of this pathogen have been recognized, but the classification of isolates into clades is timeconsuming and still sometimes leads to unclear results. We report a case of subcutaneous phaeohyphomycosis caused by a plant pathogen in a patient with acute heart failure. A new boll rot disease of cotton gossypium hirsutum l. Few studies referred to the formation of chlamydospores by c. Unlimited viewing of the articlechapter pdf and any associated supplements and. Pdf first report of corynespora cassiicola causing leaf and. On the lower surface, small, light brownish, somewhat soakedwater lesions appear first. Corynespora cassiicola is causing numerous emerging diseases, including target spot of cotton a disease is emerging if increase in host range, geographic range, or incidence is.
He found that the fungus was pathogenic to leaves of hydrangea, hydrangea macrophylla ser. Borah r k, ahmed f s, sarmah g s, bhabesh gogoi, 2012. Pdf the fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. This fungus is present in some hevea rubber plantations and nurseries in hainan and yunnan provinces of south china.
For your convenience and fast downloads you can use globus and api. Corynespora cassiicola is a species of fungus well known as a plant pathogen. Cassiicola known elsewhere causing leaf spot of cotton. Pdf purification and characterization of cassiicolin. Cca corynespora blight and target spot corynespora cassiicola gc powdery mildew golovinomyces cichoracearum ccu scab and gummosis cladosporium cucumerinum kgmmv kyuri green mottle mosaic virus cgmmv cucumber green mottle mosaic virus pcu downy mildew pseudoperonospora cubensis. Frontiers genomewide analysis of corynespora cassiicola. Diagnostic features of corynespora cassiicola and its. Purification and characterization of cassiicolin, the toxin produced by corynespora cassiicola, causal agent of the leaf fall disease of rubber tree. Ripe fruits and abaxial surfaces of the leaves were significantly more susceptible to infection than unripe fruits and adaxial surfaces of leaves. The risk corynespora cassiicola would pose to california is evaluated below.
Documents about corynespora cassiicola coryca this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Pathogenicity of corynespora cassiicola on different hosts. Corynespora cassiicola is the causal organism of the most devastating leaf disease of rubber in sri lanka. Brazil is the worlds second major producer of coffea canephora solitary or coalesce and form extensive irregular lesions. Strobilurins and related compounds are prone to the development of resistance and should be rotated with other chemistries. Although variation in aggressiveness to cotton and tomato were observed, no genetic diversity. Later, the lesions become brown, necrotic, slightly sunken, with brownreddish margins. Suppression by red light irradiation of corynespora leaf. In rest of the cultivars, the occurrence of this fungus was very poor to nil. Aug 31, 2018 corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. Target spot of tomato, caused by corynespora cassiicola, was first reported in florida in 1972. Mar 28, 2017 corynespora cassiicola was consistently isolated from the leaf spots on inoculated lettuce plants following the same method that was used for the initial isolations. Only a single, historical clinical case of human infection caused by c.
Structural analysis of cassiicolin, a hostselective. Corynespora cassiicola is causing numerous emerging diseases, including target spot of cotton a disease is emerging if increase in host range, geographic range, or incidence is observed, or new strains evolve. However, no polymorphisms were found at the its sequences. We do not use these to store personal information about you. The molecular detection of corynespora cassiicola on. Sobers the first report of corynespora cassiicola berk. Suppression by red light irradiation of corynespora leaf spot. A new record of leaf spot disease on aquilaria malaccensis lamk in india.
Structural analysis of cassiicolin, a hostselective protein toxin from corynespora cassiicola. The causal organism was isolated in pure culture and identified ascorynespora cassiicola berk. In other words, the leaf wetness is likely a major environmental factor driving the disease for this fungal pathogen. Leaf spot of pawpaw is hereby reported for the first time in nigeria. Detection of corynespora cassiicola in hevea rubber tree from. Red, lesions enlarged, coalesced, and finally covered the entire leaves of cucumber. Corynespora cassiicola, which causes corynespora leaf fall disease, is one of the most destructive and economically important fungal pathogens of hevea rubber trees in asian and african countries. Arvind kumar kurre, meghchand dewangan and dantre, r. Corynespora cassiicola berk and curt wei, incitant of leaf spot of brinjal, survives at the room temperatures 2830 c. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. Detection of corynespora cassiicola in soil with realtime quantitative pcr. Genomewide analysis of corynespora cassiicola leaf.
Corynespora cassiicola corycadocuments eppo global database. Growth sensitivity of corynespora cassiicola to thiophanate. Pdf symptomatology, hosts, and pathogenicity of corynespora. Corynespora cassiicola was pathogenic on the roots and hypocotyls of soybean seedlings grown in infested soil at ottawa. Diagnostic features of corynespora cassiicola and its associated diseases identifying c. Clonality and geographic structure of hostspecialized. The effect of red light irradiation on development of corynespora leaf spot of cucumber plants cucumis sativus l. Subcutaneous infection caused by corynespora cassiicola, a. Cotton, cowpea, cucumber, eggplant, sesame, soybean, tobacco. Among them, 12, 34, 56, and 78 were pairs of epimers. A boll rot of cotton caused by corynespora cassiicola in. Differentiation of corynespora cassiicola and cercospora.
Genetic variation of 42 isolates of corynespora cassiicola, a destructive fungal pathogen of many economically important crop plants including rubber, was. Chemical investigation of the ethyl acetate extract of corynespora cassiicola, isolated from leaf tissues of the chinese mangrove medicinal plant laguncularia racemosa, yielded four new secondary. Differentiation of corynespora cassiicola and cercospora sp. A case of phaeohyphomycosis caused by corynespora cassiicola. The planar structures were determined by extensive nmr spectroscopic data.
Two primer pairs showed specificity to corynespora cassiicola and one primer pair showed specificity to. Download fulltext pdf download fulltext pdf physiological characterization of corynespora cassiicola isolates article pdf available in tropical plant pathology 384. The organism was isolated and identified as corynespora cassiicola according to its morphological characteristics and. Initial characterization of corynespora cassiicola affecting florida tomatoes pdf. No known resistance to target spot is currently available in commercial cultivars. The symptom is characterized by a papery center surrounded by a yellow halo. Pdf effects of corynespora cassiicola on lantana camara. Pdf on sep 10, 2012, jose ricardo liberato and others published.
Transformation of corynespora cassiicola by agrobacterium. Sensitivity of corynespora cassiicola from soybean to. The absolute configurations of 110 were assigned by the modified moshers method and tddft ecd calculations. Previous studies revealed that populations from the three hosts are genetically distinct and host specialized. The isolation ofum591 from a patients contact lens indicates the pathogenic. Whole genome sequences of four cotton and one soybean isolate from tennessee were used to develop single nucleotide polymorphism markers for cotton isolates. Evaluation phylloplane fungi as biocontrol agent of corynespora lea. In the rubber tree, this fungus causes the corynespora leaf fall clf disease, which increasingly affects natural rubber production in asia and africa. Jsv provided expertise for the manual tuning of clustering parameters. Gao wei 1,2, li baoju 2, wang wanli 1, hao yongjuan 1, shi yanxia 2. Corynespora cassiicola is a destructive plantpathogenic fungus causing widespread target spot epidemics, including outbreaks on cotton, soybean, and tomato in the southeastern united states. Genomic insight into pathogenicity of dematiaceous fungus. A new leaf and berry spot disease, and leaf fall caused by corynespora cassiicola is reported on coffea canephora in brazil.
Evaluation of fungicides against target leaf spot caused by corynespora cassiicola. Growth inhibition was evaluated measuring the diameter of the. Several lineages of this pathogen have been recognized, but the classification of isolates into clades is timeconsuming and still sometimes leads to. Pathogenicity of corynespora cassiicola on different hosts in. Similarly, the phylogenetic analysis of 30 isolates of c. It is a reemerge pathogen on cotton in southeastern us. Pdf sensitivity of corynespora cassiicola from soybean to. Cotton isolates had little diversity at the genome level and very.
Pdf intraspecific variability of corynespora cassiicola. Pdf infection process of corynespora cassiicola tagged. Chemical investigation of the ethyl acetate extract of corynespora cassiicola, isolated from leaf tissues of the chinese mangrove medicinal plant laguncularia racemosa, yielded four new secondary metabolites, including three decalactones, xestodecalactones df as well as corynesidone c 4, in addition to four known compounds. Phaeohyphomycosis caused by a plant pathogen, corynespora. Frequency distribution of ec 50 values gml for thiophanatemethyl from 40 greenhousecollected isolates of corynespora cassiicola from african violet in 2007 displayed with a maximum xaxis value of 1. Corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. Initial characterization of corynespora cassiicola affecting florida tomatoes. Detection of corynespora cassiicola in hevea rubber tree. This fungus is present in some hevea rubber plantations and nurseries in. Morphological and molecular characterization of isolates. Hofs inhibit corynespora cassiicola growth in vitro and protect glycine max plants against this pathogen. Evaluation of fungicides against target leaf spot caused. Corynespora cassiicola corycaoverview eppo global database. Eco ri, msp i and rsa i endonucleases identified all isolates as corynespora cassiicola.
Two single nucleotide polymorphisms snps were detected in the internal transcribed spacer region of the nuclear ribosomal dna rdnaits of 21 corynespora cassiicola isolates obtained from a number of hevea clones grown in rubber plantations in. Two primer pairs showed specificity to corynespora cassiicola and one primer pair showed specificity to cercospora sp. Our raw reads are also published to sra at ncbi for bulk download needs. Here we confirm the occurrence of this species as an agent of subcutaneous phaeohyphomycosis. Pdf chlamydospore formation by corynespora cassiicola. Corynespora cassiicola from cotton and soybean in the southeastern united. Corynespora cassiicola appeared to be a minor pathogen for okra. Cca corynespora blight and target spot corynespora cassiicola gc powdery mildew golovinomyces cichoracearum ccu scab and gummosis cladosporium cucumerinum kgmmv kyuri green mottle mosaic virus cgmmv cucumber green mottle mosaic virus pcu downy mildew pseudoperonospora cubensis cmv cucumber mosaic virus prsv papaya ringspot virus. In the last few decades it has become one of the most destructive foliar and fruit diseases of tomato in the state mackenzie et al.
Metconazole exhibited excellent preventive and curative activities in vivo metconazole reduced disease severity of corynespora. A new foliar disease, corynespora leaf spot, has been detected in arkansas cotton travis faske extension plant pathologist corynespora leaf spot has been detected in several commercial cotton fields on a few varieties fm 1944, st 5458, and phy 499 in. Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in malaysia hsueh, 2011. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole article pdf available in tropical plant pathology 385. Pdf corynespora brown spot of papaya corynespora cassiicola. The yellowish area surrounding the lesion can be seen only against the light. Corynespora brown spot of papaya corynespora cassiicola.
A total of 651 isolates of cucumber corynespora leaf spot fungus corynespora cassiicola collected from cucumber in japan, either with 438 isolates or without 2 isolates a prior histo ry of boscalid use, were tested for their sensitivity to boscalid by using a mycelial growth inhibition method on. The fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole sheila a. Diversity of the cassiicolin gene in corynespora cassiicola and relation with the pathogenicity in hevea brasiliensis.
Transcriptome and mirna analyses of the response to. Twelve new chromone derivatives, corynechromones al 112, were isolated from the spongederived fungus corynespora cassiicola xs200900i7. Corynespora cassiicola requires high humidity for infection and is additionally favored in locations with substantial periods of high moisture 1644 hours. Phylloplane fungi % biological control % corynespora % rubber % antagonism. B hyphae with septa black arrow in the edematous papillary dermis periodic. Unlimited viewing of the articlechapter pdf and any associated supplements and figures. Continuing to use this website means you agree to our use of cookies. Extensive superficial necrotic lesions developed on seedlings from inoculated seeds planted in the field in midmay, but few symptoms appeared on seedlings planted in late may and in june. An indirect phenotyping method was developed in order to estimate the susceptibility of rubber tree clonal varieties to corynespora leaf fall clf disease caused by the ascomycete corynespora cassiicola. Corynespora cassiicola is an ascomycetes fungus with a broad host range and diverse life styles. Decalactone derivatives from corynespora cassiicola, an. Genome sequences and snp analyses of corynespora cassiicola. The isolate, which on cotton is pathogenic only to bolls, is considered to be different from a strain ofc.
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